Autophagy is the main mechanism of cell death associated to Sorafenib.

<p>a) Caspase 3/7 activity was evaluated in ACHN cells treated with Sorafenib for 24 hours. b) Viability of ACHN cells was evaluated under the same conditions by MTT assay. c) ACHN cells were exposed to 10 μM Sorafenib for 16 hours. Protein extracts were blotted with the indicated antibodies. d) Caspase 3/7 activity was evaluated in 786-O cells treated with Sorafenib for 24 hours. e) Viability of 786-O cells was evaluated by MTT assay as in B). f) 786-O cells were exposed to 10 μM Sorafenib for 16 hours. Protein extracts were blotted with the indicated antibodies. Tubulin was used as a loading control.