Wound generation and healing processes in the microfluidic devices.

<p><b>(A)</b> Schematic of wound formation and healing processes in a nano-pattern integrated microfluidic device. First, the wound is formed due to the layered flow of trypsin/EDTA. The enzyme induces cell detachment from the nano-patterned surface within a selective region. Trypsin/EDTA is replaced with culture medium after wound formation is finished. Leading cells on the wound edge recover into the cell-free area (wound healing process). <b>(B)</b> Micrographs of the wound formation and healing processes. Sequentially, from the top: the cell culture, wound formation and wound healing stages.