Nano-pattern integrated microfluidic system used to reproduce the wound healing process.

<p><b>(A)</b> CAD design of the microfluidic device, which includes 2 inlets, 1 outlet and a cell culture region. <b>(B)</b> Process used to fabricate the microfluidic channel and nano-pattern. The device and nano-patterns were irreversibly combined through plasma bonding methods. <b>(C)</b> 3D image of the combined microfluidic device with the nano-pattern. Schematic of the <i>in vitro</i> wound formation process in the microfluidic channel using the layered flow of trypsin/EDTA. Due to trypsinization, cells detached from the microfluidic channel, allowing the selective formation of a cell-free area.