Intestinal FXYD12 and sodium-potassium ATPase: A comparative study on two euryhaline medakas in response to salinity changes - Fig 5

<p><b>Confocal 3D micrographs of double immunofluorescence staining of NKA α-subunit (NKA; green; A, D) and FXYD12 (red; B, E) in the cryosection of the brackish water-acclimated Indian medaka intestines.</b> Immunosignals of NKA and FXYD12 were both detected in epithelial cell of intestinal villi. The results were similar between the cross section (<b>A-C</b>) and longitudinal section (<b>D-F</b>) of the epithelial cells. The merged image (yellow; <b>C</b>, <b>F</b>) revealed that FXYD12 colocalised to the basolateral membrane of NKA-immunoreactive cells. *, lumen. Scale bar: 10 μm.