Zp-V3 is much more responsive to BCR crosslinking than Zp-P.

<p><b>(A)</b> EBV-negative BJAB cells were transfected with a promoterless luciferase construct, or luciferase constructs driven by the Zp-P or Zp-V3 promoters, and luciferase activity was measured after 24 hours. The amount of luciferase activity for each condition (average and the standard deviation, SD) is shown. <b>(B)</b> BJAB cells were transfected with luciferase constructs driven by the Zp-P or Zp-V3 promoters, and then treated with or without anti-human IgM (10 mg/mL) 24 hours later to activate the B cell receptor (BCR). Luciferase activity was measured at 48 hours after transfection and results were normalized to that each promoter’s untreated condition (set as 1). The fold increase in luciferase activity is shown for each condition (average and SD). <b>(C)</b> EBV-negative NOKs epithelial cells were transfected with the Zp-P or Zp-V3 luciferase vectors, and a KLF4 expression vector or vector control as indicated, and luciferase activity was measured 24 hours post-transfection. The amount of luciferase activity for each condition (average and SD) is shown and results were normalized to that of each promoter’s untreated condition (set as 1).