<p>(A) Purification of fork-associated proteins by native-iPOND in response to HU (4mM– 4hours). No Click, negative control; Chase, chromatin behind the forks. (B) Western blots of the soluble and insoluble fractions of RKO cells either untreated (-) or treated with 4 mM HU for 4 hours (+). Bar graph shows Image-J quantifications of ARTEMIS and RAD51 bands normalized to ORC2 intensity. (C) Quantification by neutral comet assay of DSBs in control cells (siLUC) and in cells depleted of ARTEMIS (siARTEMIS) and treated or not for 4 hours with 4mM of HU and/or 2.5μM of DNA-PK inhibitor (NU7441). The plot shows mean tail moment (% DNA in tail x tail length). Error bars represent SEMs. At least 250 comets were scored. ***p < 0.0001 (Mann–Whitney t...
<p>(<b>A</b>) Western blot analysis of MAPK signaling by analyzing ERK1/2 and RSK phosphorylation in...
<p>(A) Foci formation of γH2AX, MDC1, 53BP1, RNF8, RAD51, BRCA1 and RPA in RNF138 deficient cells or...
<p>(A) MDA-MB-231 cells were transfected with control (luc) siRNA or JNK siRNA for 3 days prior to i...
<p>(A) Quantification by neutral comet assay of DSBs induced in response to treatment with 4 mM HU f...
<p>(A) Schematic representation of the DNA fiber assay with images of typical restarted or stalled f...
<p>(A) Western blot of whole cell extracts of RKO cells showing depletion of Mus81 by siMus81 compar...
<p>(A) Quantification by neutral comet assay of DSBs induced, in response to treatment with various ...
<p>(<b>A</b>) Assessment of protein depletion by Western blotting in GM01604 cells after transfectio...
<p>(A) G2/M checkpoint analysis in U2OS cells. Inhibition of ATM or specific siRNA depletions are as...
<p>(<b>A</b>) Immunoblot of 1AT, HRD1, BiP and Hsp90 protein expression in cell lysates following si...
<p>A, Shows western blotting and densitometric measurements (upper panel) for NRF2, RKIP and β-actin...
<p>(A) GEF-H1 mediated NF-κB reporter gene expression in the absence or presence of dominant negativ...
<p>(A–D) Sensitivity of <i>wild-type</i>, <i>ARTEMIS</i><sup>−/−</sup>, <i>LIG4</i><sup>−/−</sup>, a...
<p>(<b>A</b>) PC12 cells were transfected with pMT2 or pMT2-FLAG-MK-STYX-FLAG. 24 hr post-transfecti...
<p>A) Proliferation-DAPI assay of shControl and shRhoA NRCF over a time course of 4 days. Doubling t...
<p>(<b>A</b>) Western blot analysis of MAPK signaling by analyzing ERK1/2 and RSK phosphorylation in...
<p>(A) Foci formation of γH2AX, MDC1, 53BP1, RNF8, RAD51, BRCA1 and RPA in RNF138 deficient cells or...
<p>(A) MDA-MB-231 cells were transfected with control (luc) siRNA or JNK siRNA for 3 days prior to i...
<p>(A) Quantification by neutral comet assay of DSBs induced in response to treatment with 4 mM HU f...
<p>(A) Schematic representation of the DNA fiber assay with images of typical restarted or stalled f...
<p>(A) Western blot of whole cell extracts of RKO cells showing depletion of Mus81 by siMus81 compar...
<p>(A) Quantification by neutral comet assay of DSBs induced, in response to treatment with various ...
<p>(<b>A</b>) Assessment of protein depletion by Western blotting in GM01604 cells after transfectio...
<p>(A) G2/M checkpoint analysis in U2OS cells. Inhibition of ATM or specific siRNA depletions are as...
<p>(<b>A</b>) Immunoblot of 1AT, HRD1, BiP and Hsp90 protein expression in cell lysates following si...
<p>A, Shows western blotting and densitometric measurements (upper panel) for NRF2, RKIP and β-actin...
<p>(A) GEF-H1 mediated NF-κB reporter gene expression in the absence or presence of dominant negativ...
<p>(A–D) Sensitivity of <i>wild-type</i>, <i>ARTEMIS</i><sup>−/−</sup>, <i>LIG4</i><sup>−/−</sup>, a...
<p>(<b>A</b>) PC12 cells were transfected with pMT2 or pMT2-FLAG-MK-STYX-FLAG. 24 hr post-transfecti...
<p>A) Proliferation-DAPI assay of shControl and shRhoA NRCF over a time course of 4 days. Doubling t...
<p>(<b>A</b>) Western blot analysis of MAPK signaling by analyzing ERK1/2 and RSK phosphorylation in...
<p>(A) Foci formation of γH2AX, MDC1, 53BP1, RNF8, RAD51, BRCA1 and RPA in RNF138 deficient cells or...
<p>(A) MDA-MB-231 cells were transfected with control (luc) siRNA or JNK siRNA for 3 days prior to i...