Additional file 1: of Combination of Chymostatin and Aliskiren attenuates ER stress induced by lipid overload in kidney tubular cells

Figure A1. mRNA levels of RAS components in HK2 cells treated with palmitic acid. A. The mRNA levels of chymase, angiotensinogen, ACE in PA-treated HK2 cells with chymostatin and/or aliskiren. B. The mRNA levels of AT1R in PA-treated HK2 cells with chymostatin and/or aliskiren. Representative results of three independent experiments are shown. ATG: angiotensinogen; ACE: angiotensin converting enzyme; AT1R: angiotensin type 1 receptor; CTL, controls; PA, palmitic acid treatment group; PA + CMT, palmitic acid plus chymostatin treatment; PA + Ali, palmitic acid plus aliskiren treatment; PA + CMT + Ali, palmitic acid plus chymostatin and aliskiren treatment. * p < 0.05 compared with controls; # p < 0.05 compared with PA. (PPTX 52 kb