Semi-quantitative analysis of AMH staining during follicular development in the porcine ovary.

<p>AMH staining was quantified in recruited primordial, primary, small preantral, large preantral, small antral, large antral and preovulatory follicles using KS300 software coupled to a Zeiss image analyzer. Low OD values correspond to strong AMH immunostaining. OD values are expressed as grey units (for more details see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197894#sec005" target="_blank">Materials and Methods</a>). Values are expressed as mean ± SEM. AMH negative stroma tissue served as a control. Different characters represent significant differences in AMH staining intensity between follicle types (b–P < 0.05; c–P < 0.01).