Truncations of the titin Z-disc predispose to a heart failure with preserved ejection phenotype in the context of pressure overload - Fig 4

<p>TUNEL staining of rat cardiac tissue to identify apoptotic cells. TUNEL⁺ nuclei in WT-Sham <b>(A1)</b>, Z-Sham <b>(B1)</b>, WT-TAC <b>(C1)</b>, and Z-TAC <b>(D1)</b> rat hearts. The same sections were count-stained with α-sarcomere actin (α-SA) to show the localization of TUNEL+ nuclei in rat cardiac tissue <b>(A2-D2)</b>. <b>(E)</b> Quantification of TUNEL⁺ cardiac cells per LV section. Representative pictures of a TUNEL⁺ nucleus <b>(F1)</b> located in a cardiomyocyte that was stained for α-SA expression <b>(F2)</b>. <b>(G)</b> Quantification of TUNEL⁺ cardiomyocytes per LV section. (*: p<0.05; **: p<0.01; ***: p<0.001. Bar = 50 μm).