MiR-18a affected the relative expression of cell cycle genes.

<p>(A) The expression analysis for CCND1 and CDK6 in C2C12 cells transfected with miR-18a mimics or NC. GAPDH was used as an internal normalized reference. Data were shown as the means ± SEM (n = 3, biological samples). *, p < 0.05; **, p < 0.01. (B) Western blot data showed the protein level of CCND1 and CDK6 in C2C12 cells transfected with miR-18a mimics or NC. Tubulin served as the internal normalized reference. (C) Quantification of protein levels. The values are mean the ± SEM (n = 3).