Nonsense mediated decay and translation re-initiation factor, Tma20, reduce levels of Stn1 via the oORF.

<p>A) Saturated cultures of indicated strains were serially diluted, 5-fold, and spotted onto YEPD solid media and incubated for two days at the indicated temperatures before being photographed. B) Western blot analysis of Stn1-Myc and tubulin levels. C) <i>STN1</i> transcript levels were measured and normalized as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007523#pgen.1007523.g001" target="_blank">Fig 1E</a>. D). Dual luciferase reporter plasmids were used, as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007523#pgen.1007523.g001" target="_blank">Fig 1B</a>, to measure the effects of <i>tma20Δ</i> and <i>nmd2Δ</i> on <i>STN1</i> URS activity. In each panel a different <i>STN1</i> URS construct was tested. The points on the graph represent independent measurements. <i>P</i> values were calculated using an unpaired t-test (**) <i>P</i> < 0.01, (***) <i>P</i> < 0.001.