<p>(A) Accumulation of green fluorescence in leaves (top and middle panels) and plants (bottom panel) of <i>N</i>. <i>benthamiana</i> 16c infiltrated with 35S-GFP alone or in combination with 35S-AC4 or 35S-P19. Constructs and time points are shown at the top and left side of the images, respectively. (B) GFP mRNA and siRNA extracted from infiltrated (LL) and systemic (SL) leaves of the plants illustrated above. Constructs and time points are shown at the top and bottom of the images, respectively. M, mock-agroinfiltrated plants. rRNA represents ethidium bromide-stained ribosomal RNA shown as loading control.</p
<p>Schematic of the T-DNA binary construct containing a GFP expression cassette and a 380 bp hairpin...
<p>(A), <i>N. benthamiana</i> (3–4 leaf stage), Turkish tobacco (4-leaf stage), tomatillo (cotyledon...
(A)16-TGS plants were co-inoculated with TYLCCNV and βM2 vector containing DNA fragment of NbSAMS2 o...
<p>(A) Plants agroinfiltrated with PVX-GFP and PVX-GFP+PVX-AC4. Constructs and time points are shown...
<p>(A) Systemic spread of green fluorescence in <i>N</i>. <i>benthamiana</i> 16c plants agroinfiltra...
Plants were mock-inoculated (A) or mechanically inoculated with RNA transcripts of TCV–GFPΔCP (B–D),...
<p>(<b>A, D</b>) Visualization of green fluorescence of representative agroinfiltrated leaves. Leaf ...
<p><i>N</i>. <i>benthamiana</i> 16C plants were infiltrated at leaf L4 with pBinGFP in the additiona...
<p>ssRNA-triggered (A) and dsRNA-triggered (B) silencing assays in <i>N. benthamiana</i> plants. The...
<p><b>(A)</b> GFP-transgenic <i>N</i>. <i>benthamiana</i> 16c leaves were infiltrated with <i>A</i>....
<p>(A) Fluorescence images (5 dpi) from leaves co-infiltrated with pBinGFP and NSs gene constructs (...
<p>(A) GFP fluorescence in regions of <i>N</i>. <i>benthamiana</i> leaves after agroinfiltration of ...
<p>(<b>A</b>) GFP fluorescence in leaves of wt and 35S:dsCaM <i>Nicotiana benthamiana</i> plants co-...
<p>(<b>A</b>) Suppression of GFP silencing in <i>Nicotiana benthamiana</i> line 16c. Leaf patches we...
<p>(A) Diagram showing insertion of the GFP gene between the N and P genes of the rSYNV antigenome t...
<p>Schematic of the T-DNA binary construct containing a GFP expression cassette and a 380 bp hairpin...
<p>(A), <i>N. benthamiana</i> (3–4 leaf stage), Turkish tobacco (4-leaf stage), tomatillo (cotyledon...
(A)16-TGS plants were co-inoculated with TYLCCNV and βM2 vector containing DNA fragment of NbSAMS2 o...
<p>(A) Plants agroinfiltrated with PVX-GFP and PVX-GFP+PVX-AC4. Constructs and time points are shown...
<p>(A) Systemic spread of green fluorescence in <i>N</i>. <i>benthamiana</i> 16c plants agroinfiltra...
Plants were mock-inoculated (A) or mechanically inoculated with RNA transcripts of TCV–GFPΔCP (B–D),...
<p>(<b>A, D</b>) Visualization of green fluorescence of representative agroinfiltrated leaves. Leaf ...
<p><i>N</i>. <i>benthamiana</i> 16C plants were infiltrated at leaf L4 with pBinGFP in the additiona...
<p>ssRNA-triggered (A) and dsRNA-triggered (B) silencing assays in <i>N. benthamiana</i> plants. The...
<p><b>(A)</b> GFP-transgenic <i>N</i>. <i>benthamiana</i> 16c leaves were infiltrated with <i>A</i>....
<p>(A) Fluorescence images (5 dpi) from leaves co-infiltrated with pBinGFP and NSs gene constructs (...
<p>(A) GFP fluorescence in regions of <i>N</i>. <i>benthamiana</i> leaves after agroinfiltration of ...
<p>(<b>A</b>) GFP fluorescence in leaves of wt and 35S:dsCaM <i>Nicotiana benthamiana</i> plants co-...
<p>(<b>A</b>) Suppression of GFP silencing in <i>Nicotiana benthamiana</i> line 16c. Leaf patches we...
<p>(A) Diagram showing insertion of the GFP gene between the N and P genes of the rSYNV antigenome t...
<p>Schematic of the T-DNA binary construct containing a GFP expression cassette and a 380 bp hairpin...
<p>(A), <i>N. benthamiana</i> (3–4 leaf stage), Turkish tobacco (4-leaf stage), tomatillo (cotyledon...
(A)16-TGS plants were co-inoculated with TYLCCNV and βM2 vector containing DNA fragment of NbSAMS2 o...