MOESM7 of An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana

Additional file 7: Figure S4. PCR analyses of the T-DNA left border to determine completeness of integration of the proUBQ10:Cas9 expression cassette. a. Schematic representation of vectors integrated in the genome. Three fragments were PCR amplified. b. PCR products. The first lane (p) is the positive control with the SM destination vector as template. mCherry positive plants for four different targets were tested. Red stars indicate plants with target deletions. DNA ladder MIX was used for P1, and 1 kb ladder for P2 and P3 regions. A 404-bp genomic region from the actin gene AT2G37620 was used as the internal control for DNA quality (fourth panel labeled with CK). Samples for different gene loci are separated by lanes with DNA size markers (M). Arrows point to 1 kb for the first 3 panels and 500 bp for the fourth panel (CK)