Mapped Fis binding sites, putative promoters, and 5´ends of mRNA at the <i>nuoA</i> promoter region.

<p>(A) The sequence of the <i>nuoA</i> promoter region. The ATG start codon of the <i>nuoA</i> gene is shown in bold. The first nucleotides of the mRNA 5´ ends are written in bold and designated as N-I and N-II. The potential –10 and –35 elements of the <i>nuoA</i> promoters (P_<i>N-I</i> and P_<i>N-II</i>, respectively) are shown in grey boxes. The nucleotides in -10 and -35 box corresponding to <i>P</i>. <i>putida</i> σ⁷⁰-type promoter consensus (</p><p>TTGACC-N₁₇-TATAC/aT</p>) are indicated in bold. The Fis binding sites are shown in black brackets. The point mutations in the Fis-nuo1, Fis-nuo2, Fis-nuo3, Fis-nuo4 and -10 boxes of promoters are indicated by arrows. The oligonucleotides PP4119-fw and PP4119-4-rev were used for the construction of p9_P_nuoA1, nuo-1-fw and PP4119-3-rev were used for the construction of p9_P_nuoA2, and nuo-1-fw and PP4119-rev were used for the construction of p9_P_nuoA12. (B) Agarose-gel electrophoresis of cDNA amplified by the RACE method for the identification of the <i>nuoA</i> mRNA 5´ ends. The arrows point to the PCR products used to determine the mRNA 5´ ends.<p>