Growth of the WT, <i>vps45</i> mutants and complemented strains in inorganic and host-related iron sources.

<p>A) and B) Growth assays in liquid media with strains pre-grown in YNB+150μM BPS for 48h. After pre-growth, 1X10⁵ of iron-starved cells/mL were inoculated in iron limited conditions supplemented with inorganic iron (i.e. YNB, YNB + 150μM BPS, YNB-BPS + 10μM FeCl₃ (30°C) and + 25μM FeCl₃ (37°C), YNB-BPS + 100μM FeCl₃) and organic iron (i.e. YNB-BPS + 10μM Heme, + 2μg mL^-1 Hemoglobin, + 50μg mL^-1 Transferrin, + 0.05% sheep blood). The optical density (600nm) was recorded over a period of 5 days. C) Spot assays are shown for serial diluted WT, mutants and complemented strains in the same conditions as in liquid assays. Plates were incubated at 30°C and 37°C for 2–10 days. D) Inductively coupled plasma mass spectrometry (ICP-MS) measurements of iron content of pre-grown iron starved cells inoculated for 48h in YNB + 150μM BPS w/o 100μM FeCl₃ at 30°C and 37°C.