Additional file 1: of Enrichment of cancer stem cells via β-catenin contributing to the tumorigenesis of hepatocellular carcinoma

Supplementary experiments and routine methods. Figure S1. Spheroid formation capability of HCC cell lines. Figure S2. β-catenin expression in HepG2 cell line. Figure S3. Knockdown efficiency of siRNA transfection. Routine Methods: Immunocytochemistry (ICC) Staining, Flow-Cytometry Analysis, Protein Extraction and Western Blot. Table S1. Primary antibodies (catalog info and dilution). Table S2. HRP conjugated secondary antibodies (catalog info and dilution). Table S3. Important reagents (catalog info). Table S4. Sequence details for RNA interference experiments. (DOCX 573 kb