Additional file 9: of Reiterative use of FGF signaling in mesoderm development during embryogenesis and metamorphosis in the hemichordate Ptychodera flava

Perturbations of FGF signaling during sand-induced metamorphosis. The morphology of a wild type Spengel larva (A1) and the Spengel larvae treated with 10 μM (A2) or 20 μM (A3) of SU5402. The images were taken 2 days after treatments. The morphology of individuals after cultured for 2 days with sand containing 0.1% BSA (B1–2), 100 ng/ml (B3–4) or 200 ng/ml bFGF protein (B5–6). The Spengel larvae transformed into either Agassiz (B1, B3, B5) or juveniles (B2, B4, B6). A1-A3 and B1-B6 are shown in two different scales, according to the scale bars in A1 and B1, respectively. (PNG 5404 kb