A VP1 mutation acquired during an enterovirus 71 disseminated infection confers heparan sulfate binding ability and modulates <i>ex vivo</i> tropism - Fig 6

<p><b>Replication of EV71-VP1</b>_{<b>97R167G</b>}<b>, EV71-VP1</b>_{<b>97L167E</b>}<b>and EV71-VP197</b>_{<b>R/L167G/E</b>}<b>in 2D (A) and 3D (B) neural tissues and HS/SCARB2 expression in the same 2D tissues (C). (A-B)</b> Viral load was measured by RT-qPCR and expressed as the mean (±SEM). (N = 2). ****P<0.0001, ⁺⁺P<0.01 or ^#P<0.05. (⁺relative to EV71-VP1_97L167E and ^# relative to EV71-VP1_97R/L167G/E at the same time point). The frequency plots are shown on the right (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007190#ppat.1007190.g003" target="_blank">Fig 3</a> for details) <b>(C)</b> 2D neural cultures were labeled by immunofluorescence. SCARB2 is stained in green, HS in red and cell nuclei in blue. Scale bar = 20 μm.