A VP1 mutation acquired during an enterovirus 71 disseminated infection confers heparan sulfate binding ability and modulates <i>ex vivo</i> tropism - Fig 4

<p><b>Replication of EV71-VP1</b>_{<b>97R167G</b>}<b>, EV71-VP1</b>_{<b>97L167E</b>}<b>and EV71-VP1</b>_{<b>97R/L167G/E</b>}<b>in reconstituted airway epithelia developed from the upper (A) and lower (B) respiratory tract and HS/SCARB2 expression in the same tissues (C -D)</b>. <b>(A-B)</b> Viral loads were measured by RT-qPCR 5 dpi in the apically collected washes of the apically or basally inoculated tissues and are expressed as the mean ±SEM (N≥2). ***P<0.001, **P<0.01, *P<0.05. Frequency plots are shown on the right (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007190#ppat.1007190.g003" target="_blank">Fig 3</a> for details) <b>(C-D)</b> Tissue sections were colored with hematoxylin and eosin (upper panel) or labeled by immunofluorescence. SCARB2 is stained in green, HS in red and cell nuclei in blue. Scale bar = 20 μm.