SL- and RQ,SL-expressing NMJs have enhanced mEPSPs.

<p><b>(A)</b> Electrophysiological traces of spontaneous activity at WT- and RQ,SL-expressing NMJs. Example traces with two different scales show variable severity of spontaneous neurotransmission phenotypes, in terms of frequency severity (left) or amplitude severity (right). <b>(B)</b> Effects of genotype on average spontaneous mEPSP amplitude (** <i>p</i> < 0.01 vs. <i>w</i>^<i>1118</i> by one-way ANOVA with Tukey’s post-hoc; ### <i>p</i> < 0.001 vs. WT by one-way ANOVA with Tukey’s post-hoc. <b>(C)</b> Effects of genotype on spontaneous mEPSP frequency. ## <i>p</i> < 0.01 vs. WT by one-way ANOVA with Tukey’s post-hoc; <i>n</i> ≥ 12 NMJs, all genotypes. <b>(D)</b> Box and whisker plots of mEPSP amplitude range at 0.5 mM extracellular [Ca²⁺]. Box denotes 25^th-75^th percentile; line denotes median; + sign denotes average; whiskers range from 1^st-99^th percentile; individual data points outside the 1^st and 99^th percentiles are plotted; (*** <i>p</i> < 0.001 by Kruskal-Wallis ANOVA with Dunn’s post-hoc vs. either <i>w</i>^<i>1118</i> or WT; <i>n</i> > 1400 mEPSPs for each genotype). <b>(E)</b> Cumulative probability histogram of the data in (D) showing a marked rightward shift in mEPSP amplitudes for SL- and RQ,SL-expressing NMJs. <b>(F)</b> Box and whisker plot (as in (D)) of mEPSP amplitude at 0.4 mM extracellular [Ca²⁺] with and without the Ca_V2.1 channel modifier BHQ (*** <i>p</i> < 0.001 by Kruskal-Wallis ANOVA with Dunn’s post-hoc vs. identical genotype +/- BHQ; <i>n</i> > 985 mEPSPs for each genotype). <b>(G)</b> Cumulative probability histogram of a subset of data in (F). 5 μM BHQ causes a partial leftward shift in the distribution of events for SL- and RQ,SL-expressing NMJs while not affecting WT-expressing NMJs. <b>(H)</b> Box and whisker plot (as in (D)) of mEPSP amplitude range when expressing the RQ,SL transgene for acute periods of developmental time (*** <i>p</i> < 0.001 by Kruskal-Wallis ANOVA with Dunn’s post-hoc vs. RQ,SL 0 hr., ### <i>p</i> < 0.001 by vs. RQ,SL 24 hr.; <i>n</i> > 1095 mEPSPs for each genotype). <b>(I)</b> Cumulative probability histogram of the data in (H) showing a rightward shift in mEPSP amplitudes for longer periods of RQ,SL expression.