The effect of PEP on MMP-2 regulatory proteins at short (3 days) and long (10 days) term incubation with PEP.

<p>Fibroblasts were incubated with PEP (1 mg/ml) or left untreated (control) for 3 and 10 days before cell medium and cell fractions were collected. ELISA analysis of the cell fraction for determining MT1-MMP (A) and the conditioned medium for determining TIMP2 (B) protein levels at day 3 and day 10. Data are presented as mean ± SEM from three independent cell experiments seeded out in triplicates. (**<i>p</i><0.01 and ***<i>p</i><0.001 by unpaired two-tailed <i>t</i>-test).