Relative affinities of ER-ESCAPE motifs for cargo receptor by analyses of GH steady-state levels.
- Ying Yin (176992)
- Mekka R. Garcia (5607710)
- Alexander J. Novak (5607713)
- Allison M. Saunders (5607716)
- Raira S. Ank (5607719)
- Anna S. Nam (2434666)
- Larry W. Fisher (445576)
DOIAbstract
<p>(A) Pre-confluent cells were separately transfected with expression plasmids encoding human GH with 60 different ER-ESCAPE motifs as noted. Eighteen hr posttransfection, cells were washed, extracted with lysis buffer, and analyzed by GH ELISA. GH values (ng GH/mg protein) were normalized within each experiment to the amount of IPV-GH. Each histogram bar represents mean ± SEM. of at least three independent transfections for each construct. (Means of triplicate GH ELISA analyses were used for each extract). Larger error bars among poorest ER-ESCAPE motifs may reflect a variable amount of aggregate formation by larger amounts of accumulated GH. Note that Φ-P-Φ ER-ESCAPE motifs are among the most efficient at trafficking GH, while those incl...
Add to ORKG