<p>Luciferase reporter assays of constructs in which the 3’ UTR of DNAJB1 is linked to Renilla luciferase were performed. Firefly was present on the same plasmids and was used for normalization. (A) The binding site of miR-543 on the 3’ UTR of DNAJB1 was mutated. (B) The two binding sites of miRNA-370 on the 3’ UTR of DNAJB1 mRNA were mutated. Columns represent mean values +/- SD of n = 27 samples, p < 0.0001.</p
<p>(A) Schematic representation of firefly luciferase reporter construct containing β-actin 3′ UTR w...
<p>A, Putative miR-143-binding site in 333 bp of PTGS2 3′ UTR construct cloned into pMIR-REPORT™ for...
<p>(<b>A</b>) Region of the MITF-M 3’UTR where miR-155 is predicted to bind (subscript numbers indic...
<p>(A) Targetscan prediction of miRNAs targeting within the first 700bp of the DNAJB1 3’-UTR. (B) Sc...
<p>A) Diagram of luciferase reporter constructs. The predicted MRE, wild-type or MRE-deleted AP-2α 3...
<p>A. 3′UTRs for 13 genes were inserted into the 3′UTR of firefly luciferase and tested against 11 d...
<p>Transient transfection analysis for luciferase expression in A549 cells co-transfected with pre-m...
<p>(A) Schematic representation of the luciferase reporter constructs, indicating the VEGFA 3′-UTR f...
<p>Luciferase reporter assay of NCI-H929 cells co-transfected with pLightSwitch_3′UTR Reporter Vecto...
<p>The analysis of the relative luciferase activity in PK-15 cells following transfection with candi...
<p>(A) HEK293T cells were transfected with a pool of miRNA mimics (hsa-miR-374a-5p, hsa-miR-542-3p, ...
<p>(A) Schematic representation of different Nrf2 3′ UTR luciferase reporters used in the transfecti...
<p>A. Luciferase reporter constructs with wild-type or mutated (for miR-30-3p binding sites) BAFF 3′...
<p>Luciferase constructs expressing the <i>Renilla</i> luciferase gene under the control of the corr...
(A) The sequences of Renilla and firefly luciferase under regulation of Dicer1, HRB and HIVEP2 3′UTR...
<p>(A) Schematic representation of firefly luciferase reporter construct containing β-actin 3′ UTR w...
<p>A, Putative miR-143-binding site in 333 bp of PTGS2 3′ UTR construct cloned into pMIR-REPORT™ for...
<p>(<b>A</b>) Region of the MITF-M 3’UTR where miR-155 is predicted to bind (subscript numbers indic...
<p>(A) Targetscan prediction of miRNAs targeting within the first 700bp of the DNAJB1 3’-UTR. (B) Sc...
<p>A) Diagram of luciferase reporter constructs. The predicted MRE, wild-type or MRE-deleted AP-2α 3...
<p>A. 3′UTRs for 13 genes were inserted into the 3′UTR of firefly luciferase and tested against 11 d...
<p>Transient transfection analysis for luciferase expression in A549 cells co-transfected with pre-m...
<p>(A) Schematic representation of the luciferase reporter constructs, indicating the VEGFA 3′-UTR f...
<p>Luciferase reporter assay of NCI-H929 cells co-transfected with pLightSwitch_3′UTR Reporter Vecto...
<p>The analysis of the relative luciferase activity in PK-15 cells following transfection with candi...
<p>(A) HEK293T cells were transfected with a pool of miRNA mimics (hsa-miR-374a-5p, hsa-miR-542-3p, ...
<p>(A) Schematic representation of different Nrf2 3′ UTR luciferase reporters used in the transfecti...
<p>A. Luciferase reporter constructs with wild-type or mutated (for miR-30-3p binding sites) BAFF 3′...
<p>Luciferase constructs expressing the <i>Renilla</i> luciferase gene under the control of the corr...
(A) The sequences of Renilla and firefly luciferase under regulation of Dicer1, HRB and HIVEP2 3′UTR...
<p>(A) Schematic representation of firefly luciferase reporter construct containing β-actin 3′ UTR w...
<p>A, Putative miR-143-binding site in 333 bp of PTGS2 3′ UTR construct cloned into pMIR-REPORT™ for...
<p>(<b>A</b>) Region of the MITF-M 3’UTR where miR-155 is predicted to bind (subscript numbers indic...