Immunization for EAE decreased the number of CD4+ and CD8+ SP TCRαβ^hi thymocytes more prominently in DA than in AO rats.

<p><b>(A)</b> Scatter plots indicate the frequency of CD4+ and CD8+ TCRαβ^hi single positive (SP) thymocytes of non-immunized and immunized for EAE DA and AO rats (gating strategy is displayed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201848#pone.0201848.s003" target="_blank">S3A and S3B Fig</a>). <b>(B)</b> Representative flow cytometry contour plots show Ki-67 staining of CD4+ and CD8+ SP TCRαβ^hi thymocytes of non-immunized and immunized for EAE DA and AO rats (gating strategy is displayed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0201848#pone.0201848.s002" target="_blank">S2C and S2D Fig</a>). Scatter plots indicate the frequency of Ki-67+ cells within CD4+ and CD8+ SP TCRαβ^hi thymocytes from non-immunized and immunized for EAE DA and AO rats. <b>(C)</b> Scatter plots indicate the number of CD4+ and CD8+ SP TCRαβ^hi thymocytes in non-immunized and immunized for EAE DA and AO rats. Two way ANOVA showed significant interaction between the effects of strain and immunization for the frequency of CD4+ (F_(1,20) = 55.12, p<0.001) and CD8+ SP TCRαβ^hi (F_(1,20) = 20.97, p<0.001) thymocytes, and the number of CD4+ (F_(1,20) = 229.8, p<0.001) and CD8+ SP TCRαβ^hi (F_(1,20) = 64.32, p<0.001) thymocytes. Data points, means and ± SD are from one of two experiments with similar results (n = 6). * p<0.05; ** p<0.01; *** p<0.001.