PSEN1 cKO mice are susceptible to EAE.

<p>WT and PSEN1 cKO mice were immunized with MOG35-55 to induce active EAE. A. Clinical course of EAE. B. Peak clinical score of EAE mice. C. Cumulative EAE scores for DMSO and GSI-treated animals. T cell cytokine expression in CNS-infiltrating CD4+ T cells from DMSO or GSI-treated mice following the Peak of EAE. On day 17 post-immunization, CNS cells were isolated and intracellular cytokine staining performed. D. Expression of IL-17 and IFNγ. E. Expression of GM-CSF and IFNγ. Distribution of the percentages of cells expressing IFNγ (F), IL-17 (G), GM-CSF (H) or FoxP3 (I) among CNS-infiltrating CD4+ T cells. Symbols indicate the percentage of cells from individual mice. Open circles indicate results from WT and filled squares indicate PSEN1 cKO mice. Error bars represent SEM. Asterisks indicate significant differences (* p<0.05, ** p<0.01 and ***p <0.001).