CCL5 levels in experimental human BBB models.

<p>CCL5 expression in the abluminal culture media M2 <b>(A)</b>, in hCMEC/D3 cells (D3) <b>(B)</b>, in U87 cells seeded on the external side of the insert (U87i) <b>(C)</b> and in U87 cells seeded on bottom wells (U87w) <b>(D)</b>. PBMCs were extracted from three groups of patients: control patients (n = 11), mild AD patients (n = 13), and moderate AD patients (n = 7). Chemokines were analyzed by the 5-plex Luminex^® xMAP^® assay containing a mixture of beads specific for each chemokine as described in Methods. Chemokine levels in lysates are expressed in pg/mg protein, and in pg/mL for culture media. The mean is represented by a colored line following PBMCs origins (blue, purple, red for control, mild AD, and moderate AD patients, respectively). *<i>P</i> < 0.05 in BBB model with PBMCs from moderate AD patients compared to BBB model with PBMCs from mild AD patients by a Mann-Whitney’s test.