Study of anaesthetic effects of clove oil (Eugenia caryophyllata) in common carp (Cyprinus carpio) under various pH and temperature condition

The anaesthetic effects of clove oil in juvenile common carp (8-12 g) were investigated under various water quality conditions, consisting of different pH and temperature. The time to loss the equilibrium, induction of anesthesia, equilibrium recovery and response recovery were measured. The results showed that clove oil induced the desirable anaesthetic condition in less than 3 minutes in all experiments. However the time of equilibrium recovery and response recovery were longer than 5 minutes in many cases. When using clove oil at concentration of 100ppm, the most suitable anaesthetic effects and recovery times were observed at water temperature of 25֯ C and pH=7 and 8,as well as at 30֯ C with pH=9. No morbidity or mortality occurred during induction of anesthesia by clove oil and recovery of fishes