Stable Physically Adsorbed Coating of PolyVinyl Alcohol\ud for the Separation of Basic Proteins1.

In aqueous capillary electrophoresis, the electroosmotic flow can be strongly suppressed by coat�ing the inner surface of the capillary. In the present work hydrophilic coating of 4% polyvinyl alcohol (PVA) as been used for the analysis of basic proteins. The coating is simple and easy to obtain. The separation of ribonuclease and α�chymotrypsin has been uniquely done with other three basic proteins (lysozyme, cyto� chrome�c and trypsin) using a buffer 11.60 mM sodium acetate and 18.40 mM acetic acid at pH 4.5 in addi�tion to positive power supply of 20 kV at 25°C. Detection was performed using UV detector at 230 nm. The proposed PVA coated capillary provides reproducible separation of five basic proteins within 10 min with RSD values for mobility bellow 1.4% (n = 6) for all the five basic proteins. The stability of coated capillary has been checked up to 40 runs. The viscosity measurement for 4% PVA have been studied and scanning elec�tron microscope (SEM) images obtained to make it compatible with future micro�chip applications