New fungi for whole-cell biotransformation of carvone enantiomers. Novel p-menthane-2,8,9-triols production

The microbial biotransformation of carvone enantiomers by Lasiodiplodia theobromae, Trichoderma harzianum and Mucor circinelloides was investigated. Biotransformation experiments were conducted using growing or resting fungi cells and the products were analyzed by GC–MS or LC–MS. The isolated compounds were identified by NMR. (S)-Carvone yielded only the enone reduction product dihydrocarvone by M. circinelloides, while (R)-carvone yielded p-menthane-2,8,9-triols when biotransformed by L. theobromae and M. circinelloides. These p-menthanetriols are being reported for the first time as biotransformation products. Neodihydrocarveol was the only product from the biotransformation of (R)-carvone by T. harzianum. These biotransformations can find practical applications, especially in the case of the dihydrocarveol production