Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses
- Kurta, R.
- Donatelli, J.
- Yoon, C.
- Berntsen, P.
- Bielecki, J.
- Daurer, B.
- DeMirci, H.
- Fromme, P.
- Hantke, M.
- Maia, F.
- Munke, A.
- Nettelblad, C.
- Pande, K.
- Reddy, H.
- Sellberg, J.
- Sierra, R.
- Svenda, M.
- van der Schot, G.
- Vartanyants, I.
- Williams, G.
- Paulraj, L.
- Aquila, A.
- Zwart, P.
- Mancuso, A.
DOIAbstract
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our re...
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