Activation of toll-like receptor-2 by glycosylphosphatidylinositol anchors from a protozoan parasite

Glycosylphosphatidylinositol (GPI) anchors and glycoinositolphospholipids (GIPLs) from parasitic protozoa have been shown to exert a wide variety of effects on cells of the host innate immune system. However, the receptor(s) that are triggered by these protozoan glycolipids has not been identified. Here we present evidence that Trypanosoma cruzi-derived GPI anchors and GIPLs trigger CD25 expression on Chinese hamster ovary-KI cells transfected with CD14 and Toll-like receptor-2 (TLR-2), but not wild-type (TLR-2-deficient) Chinese hamster ovary cells. The protozoan-derived GPI anchors and GIPLs containing alkylacyl-glycerol and saturated fatty acid chains or ceramide were found to be active in a concentration range of 100 nM to 1 muM. More importantly, the GPI anchors purified from T. cruzi trypomastigotes, which contain a longer glycan core and unsaturated fatty acids in the sn-2 position of the alkylacylglycerolipid component, triggered TLR-2 at subnanomolar concentrations. We performed experiments with macrophages from TLR-2 knockout and TLR-4 knockout mice, and found that TLR-2 expression appears to be essential for induction of IL-12, TNF-alpha, and NO by GPI anchors derived from T. cruzi trypomastigotes. Thus, highly purified GPI anchors from T. cruzi parasites are potent activators of TLR-2 from both mouse and human origin. The activation of TLR-2 may initiate host innate defense mechanisms and inflammatory response during protozoan infection, and may provide new strategies for immune intervention during protozoan infections. The Journal of Immunology, 2001.Univ Fed Minas Gerais, Inst Biol Sci, Dept Biochem & Immunol, Belo Horizonte, MG, BrazilOswaldo Cruz Fdn, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, BrazilUniv Sao Paulo, Dept Parasitol, Sao Paulo, BrazilOsaka Univ, Dept Host Dev, Microbial Dis Res Inst, Osaka, JapanUniv Fed Sao Paulo, Unit Expt Oncol, Sao Paulo, BrazilBoston Univ, Sch Med, Boston, MA 02118 USAUniv Fed Sao Paulo, Unit Expt Oncol, Sao Paulo, BrazilWeb of Scienc