Torque Teno Virus (TTV) es Prevalente en Primates No Humanos Brasileños y Pollos (Gallus gallus domesticus)

Torque teno virus (TTV) is an infectious agent of worldwide distribution isolated by the first time as the agent of an acute post-transfusion hepatitis in a patient in Japan. It has been classified into a new floating genus called Anellovirus. Recent studies showed that TTV can also be identified in serum specimens obtained from domesticated farm animals and from non-human primates. To better understand the relationship between TTV and their hosts, a study to detect virus in the serum and whole blood of Brazilian non-human primates and in the plasm of chickens was performed by applying the PCR-UTR-A technique, followed by a genomic sequence and phylogenetic analysis. By nested-PCR-UTR, the DNA of TTV was detected in sera from 4 (5.3%) of 75 Cebus apella, 2 (40%) of 5 Alouata fusca, 1 (20%) of 5 Alouata caraya, 1 (5.2%) of 19 Callithrix penicilata, 1 (4%) of 25 Callithrix jacchus, 1 ( 20%) of 5 Saimiri sciureus and 1 (25%) of 4 Leontopithecus chrysomelas. Phylogenetic analysis revealed that sequences detected in 8 samples clustered with TTV sequences So-TTV2 (Saguinus oedipus) and At-TTV3 (Aotes Trivirgatus). Three sequences showed similarity with a human Torque Teno Minivirus (TLMV). TTV ORF2 DNA was detected in one sera sample and one whole blood sample of non-human primates and in one plasm sample of chicken. Phylogenetic analysis revealed that the sequences amplified by the ORF2 region show no difference between human, non-human primates and chicken. This is the first report of TTV in brazilian new world non-human primates and chicken.Biol Inst Sao Paulo, Electron Microscopy Lab, BR-04014002 Sao Paulo, BrazilFundacao Hemoctr Sao Paulo, Sao Paulo, BrazilUniv Fed Sao Paulo, Lab Retrovirol, Sao Paulo, BrazilUniv Sao Paulo, Inst Biomed Sci, BR-05508 Sao Paulo, BrazilInst Adolfo Lutz Registro, Sao Paulo, BrazilEcol Pk Tiete, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg Tech, Sao Paulo, BrazilUniv Fed Sao Paulo, Lab Retrovirol, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg Tech, Sao Paulo, BrazilWeb of Scienc