Development and analysis of anaerobic biofilms onto hydrophobic and hydrophilic surfaces

Fluorescent in situ hybridization (FISH) with domain and group specific probes that target intracellular 16S rRNA were used to investigate microbial composition of anaerobic biofilms; developed on polypropylene (hydrophobic) and glass (hydrophilic) surfaces fitted inside a Modified Robbins Device (MRD). Crushed anaerobic granular sludge was used as inoculum for biofilm development in the NMD. The inoculum and biofilms formed showed nearly the same microbial composition, both were dominated by hydrogenotrophic methanogenic Archaea related to the Methanobacteriaceae as detected by the specific probe (MB1174). This group accounted for 44 to 90% of the DAPI-stained cells. Cells which hybridized to the Bacteria specific probe (EUB338) accounted for 3-18% of the DAPI-stained cells. After the first day of the biofilm formation experiment, a larger number of cells, 4.6 x 10(4) cells mm(-2), could be seen colonizing the polypropylene coupon compared to the glass, 8.2 x 10(3) cells mm(-2). However, after 9 days these numbers were very similar, i.e. 6.3 x 10(5) cells mm(-2) and 7.2 x 10(5) cells mm(-2), for the glass and polypropylene coupons, respectively. Our data suggest that the hydrophobicity of the support material did not influence the initial development and the microbial composition of anaerobic biofilms developed in the MRD.Univ Sao Paulo, Dept Microbiol, Lab Microbiol Ambiental, BR-05508900 Sao Paulo, BrazilUNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, BrazilUSP, Escola Engn Sao Carlos, Dept Hidraul & Saneamento, BR-13566590 Sao Carlos, SP, BrazilUNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, BrazilWeb of Scienc