Immunoexpression of DIABLO, AIF and Cytochrome c in Gastric Adenocarcinoma Assessed by Tissue Microarray

The aim of this study was to analyze the immunoexpressioh of (Smac) DIABLO, AIF, cytochrome c, Ki-67 and cleaved caspase-3 in gastric cancer. A tissue microarray (TMA) paraffin block was constructed using gastric adenocarcinoma tissue and adjacent normal adjacent mucosa from 87 patients who had not previously undergone radiotherapy or chemotherapy. Immunohistochemistry was used to evaluate the protein levels. Samples were positive for (Smac) DIABLO in 37 (45.6%) and 37 (46.8%), for AIF in 31 (36.9%) and 36 (45.6%), for cytochrome c in 60 (68.9%) and 44 (54.4%), for Ki-67 in 63 (72.4%) and 52 (61.9%) and for cleaved caspase-3 in 21 (24.1%) and 3 (3.4%) cases of tumor and adjacent normal tissues, respectively. Our results suggest that increased expression of Ki-67 and cleaved caspase-3 could contribute to carcinogenesis. The expression of these proteins indicates an attempt of cells to maintain tissue homeostasis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Paulo, UNIFESP, Dept Pathol, BR-11060001 Santos, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Med, Div Gastroenterol, BR-11060001 Santos, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Pathol, BR-11060001 Santos, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Med, Div Gastroenterol, BR-11060001 Santos, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, BrazilFAPESP: 2010/15764-8Web of Scienc