Prevalence rate of white spot syndrome virus (WSSV) in farmed white leg shrimp (Litopenaeus vannamei) in Bushehr province

We surveyed presence of white spot syndrome virus (WSSV) in farmed white leg shrimp in Bushehr province with assumed a prevalence of 2% of virus in target population. Hence, 468 samples were collected in two separate phases from May to October 2006. In the first phase, 200 samples (each sample was 150 pieces of post larvae with average age 7 days) were taken from 3 active hatcheries and in the second phase, 268 samples from 418 ponds in 5 sites were collected. Samples were tested by "Nested PCR" for detection of WSSV with 1Q2000 commercial kits. Results were negative and with respect to sampling method and sensitivity and specifity of Nested PCR we concluded that cultured shrimps were free of WSSV in 2006 in Bushehr province