Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli, is arguably one of the most devastating diseases of sugarcane. Four diagnostic techniques were compared for 100 fields of sugarcane (Saccharum interspecific hybrids) of unknown infection status. These were quantitative polymerase chain reaction on pooled leaf sheath biopsies (LSB-qPCR), conventional PCR on the same templates (LSB-PCR), evaporative-binding enzyme immunoassay (EB-EIA) coupled with phase contrast microscopy (PCM) on expressed xylem sap from the same fields, and conventional PCR on the same xylem sap samples. LSB-qPCR and LSB-PCR detected the causal agent in 27 and 18 fields, respectively, whereas, from samples of expressed xylem sap from the sam...
Cloned DNA's from the region between the 16S rRNA and 23S rRNA genes of #Clavibacter xyli# subsp. #x...
A sensitive, specific polymerase chain reaction-based assay was developed for the detection of the c...
permits unrestricted use, distribution, and reproduction in any medium, provided the original work i...
Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli, is arguably one o...
The ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is one ...
Leifsonia xyli subsp xyli (Lxx) is the etiological agent of ratoon-stunting disease (RSD), one of th...
A NEW MOLECULAR RSD diagnostic platform was developed using polymerase chain reaction (PCR) on poole...
A new molecular RSD diagnostic platform was developed using polymerase chain reaction (PCR) on poole...
Ratoon stunting disease (RSD) caused by Leifsonia xyli subsp. xyli is a major constraint to sugarcan...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
To our knowledge, this is the first report that Leifsonia xyli subsp. xyli, previously named Claviba...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
Leifsonia xyli subsp. xyli, causal agent of ratoon stunting disease (RSD) of sugarcane (Saccharum in...
Cloned DNA's from the region between the 16S rRNA and 23S rRNA genes of #Clavibacter xyli# subsp. #x...
A sensitive, specific polymerase chain reaction-based assay was developed for the detection of the c...
permits unrestricted use, distribution, and reproduction in any medium, provided the original work i...
Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli, is arguably one o...
The ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is one ...
Leifsonia xyli subsp xyli (Lxx) is the etiological agent of ratoon-stunting disease (RSD), one of th...
A NEW MOLECULAR RSD diagnostic platform was developed using polymerase chain reaction (PCR) on poole...
A new molecular RSD diagnostic platform was developed using polymerase chain reaction (PCR) on poole...
Ratoon stunting disease (RSD) caused by Leifsonia xyli subsp. xyli is a major constraint to sugarcan...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
To our knowledge, this is the first report that Leifsonia xyli subsp. xyli, previously named Claviba...
The availability of efficient diagnostic methods is crucial to monitor the incidence of crop disease...
Leifsonia xyli subsp. xyli, causal agent of ratoon stunting disease (RSD) of sugarcane (Saccharum in...
Cloned DNA's from the region between the 16S rRNA and 23S rRNA genes of #Clavibacter xyli# subsp. #x...
A sensitive, specific polymerase chain reaction-based assay was developed for the detection of the c...
permits unrestricted use, distribution, and reproduction in any medium, provided the original work i...