Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS)

Weeks TJ, Huser T. Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS). Journal of Visualized Experiments (JoVE). 2010;(44):e2085.A combination of three single wavelength short-pulsed lasers is used to generate coherent anti-Stokes Raman scattering (CARS) and doubly-resonant CARS (DR-CARS). The difference between these signals provides enhanced sensitivity for otherwise difficult to detect coherent Raman signals, enabling imaging of weak Raman scatterers