Denaturation map of the circular mitochondrial genome of Neurospora crassa

Bernard U, Pühler A, Mayer F, Kuntzel H. Denaturation map of the circular mitochondrial genome of Neurospora crassa. Biochim Biophys Acta. 1975;402(2):270-278.A denaturation map of mitochondrial DNA from the wild type strain 5256 of Neurospora crassa was constructed by computer analysis of the contour length distribution of single- and double-stranded regions of nineteen circular and three full length linear molecules after partial denaturation. The data suggest that mitochondrial DNA in this strain is a homogeneous population of a circular molecule of molecular weight 41 - 10(6) with an asymmetric distribution of AT-rich regions, and that linear molecules derive from this genome by random breaks during isolation