First microsatellite loci for spectacled salamanders (Salamandrina perspicillata and S. terdigitata) endemic to the Apennine peninsula

Hauswaldt JS, Polok A, Angelini C, Steinfartz S. First microsatellite loci for spectacled salamanders (Salamandrina perspicillata and S. terdigitata) endemic to the Apennine peninsula. Conservation Genetics Resources. 2012;4(2):399-402.We describe ten microsatellite loci isolated for the oldest lineage of extant salamandrids, the spectacled salamanders (Salamandrina), a genus of terrestrial salamanders endemic to the Apennine peninsula (Italy). Primers were tested in 24 individuals per species, the northern species (Salamandrina perspicillata) and the southern species (Salamandrina terdigitata). All loci were polymorphic in S. perspicillata with number of alleles ranging from three to eleven and observed heterozygosity ranging from 0.25 to 0.83. In S. terdigitata eight loci were polymorphic, the number of alleles ranged from two to nine, and observed heterozygosity from 0.04 to 0.92