Pengaruh Ph, Suhu, dan Reusability dalam Matriks Kitosan Mg Terhadap Aktivitas Immobilisasi Enzim Papain

Immobilization of papain enzyme is a process where by enzymes are physically placed in a certain place or space so that their catalytic activity remains and can be used repeatedly. In this immobilized papain enzyme study the matrix used was chitosan-Mg, its activity tested to be compared with pure papain used by pH and temperature. The enzyme activity of papain 2.5 mL phosphate buffer plus 0.5 mL casein with variation of pH 4,5,6,7, and 8 was put into reaction tube 1. The reaction tube 2 was filled by 0.25 mL papain enzyme. In preincubation for 10 minutes with temperature variations 50,60,70, and 80°C. Test tube 2 was poured into test tube 1, re-incubated for 10 minutes. Added 1 mL TCA 10%, cooled 10 minutes, centrifugation 3000rpm 10 minutes. Taken 2 mL filtrate plus 1 mL of distilled water, 4 mL Na₂CO₃, 1 mL of follin reagent measured its absorbance by spectrophotometer with wave =650 nm. The enzyme activity of papain occurs optimum pH at pH 7 in the immobilized papain. Maximum activity in the immobil papain enzyme occurs at 70°C. Reusabillity of immobil papain enzyme can be used 5 times