Glucose-Neopentyl Glycol (GNG) amphiphiles for membrane protein study

The development of a new class of surfactants for membrane protein manipulation, "GNG amphiphiles", is reported. These amphiphiles display promising behavior for membrane proteins, as demonstrated recently by the high resolution structure of a sodium-pumping pyrophosphatase reported by Kellosalo et al. (Science, 2012, 337, 473).This work was supported by NIH grant P01 GM75913 (S.H.G.), the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2008-0061856 to P.S.C., K.H.C.), NS28471 (B.K.), the European Community's Seventh Framework Programme FP7/2007-2013 under grant agreement no. HEALTH-F4-2007-201924, EDICT Consortium (B.B., K.G., U.G.), the Danish National Research Council (C.J.L., U.G.), the Lundbeck Foundation (S.G.F.R., C.J.L., U.G.). R.R. was funded by the Defence, Science and Technology Laboratories (DSTL), Porton Down, UK. We thank Philip Laible (Argonne National Laboratory) for supplying membrane preparations from R. capsulatus. We also thank Chiara Lee and David Drew (Imperial College London), and Jonathan Ruprecht (Medical Research Council-Mitochondrial Biology Unit, Cambridge) and Gary Cecchini (University of California, San Francisco) for providing purified GlpG and SQR samples, respectively