ANALYSIS OF SUGAR-CHAIN IN STERSS-COPING HUMORAL LIPOIDS

Stress-coping for keeping adaptation is the nature of animals. Mice given various stresses produces stress-coping substances. Mouse given forced swimming stress produces humoral lipoids preventing death of mouse given repeated immunization stress in the lipoid fractions eluted with 100mM NaCl and with 250 mM NaCl in DE-52 anion-exchange chromatography. The lipoids are not identified, however, cerebroside sulfates fractionated with 100 mM NaCl and with 250 mM NaCl is also reported to prevent mouse death induced by the repeated immunization stress. Cerebroside sulfates have sugar-chains, but analysis of the sugar-chain is difficult because of the sulfation. The stress-coping cerebroside sulfates and the stress-coping humoral lipoids were desulfated using silyl-agents and dehydrated pyridine, and were analysed the sugar-chain reactivity using lectin-ELISA method. Galbeta1-4GlcNAc reactivity was increased in the fraction eluted with 100mM NaCl of cerebroside sulfates and in that of the mouse humoral lipoids. Fucalpha1-3 reactivity was increased in the fraction eluted with 250 mM NaCl of cerebroside sulfates and in that of the humoral lipoids. These suggest stress-coping activity of the cerebroside sulfates and the mouse humoral lipoids would be relating to the sugar-chain Galbeta1-4GlcNAc or Fucalpha1-3