Pharmacological and Toxicological Studies of Methanolic Extract of Mikania cordata

This thesis submitted in partial fulfillment of the requirements for the degree of Masters of Pharmacy (M.Pharm) in East West University, Dhaka, Bangladesh.Purpose: The research work was carried out to determine the pharmacological activities of methanolic extract of Mikania cordata. Method: Methanolic extract was administered orally to the animal model (Swiss albino) and the effects were determined by comparing with respect to control group which were treated with 5% CMC. For every experiment positive control was used. Different experiments were used to determine the pharmacological profile which was collected from internationally published publications and journals. Result: The CNS activity was evaluated by open field method and hole board test. In the open field method and hole board experiment the crude extract of Mikania cordata. (200mg/kg, & 400mg/kg) dose dependently reduces the number of peripheral locomotion, central locomotion and leaning in the open field test and reduces the number of head dipping and head poking in the hole board test. The reduction is significant when it is compared to the standard drug. The aim of the study was also to investigate the possible toxicity of the plant Mikania cordata and especially to establish the safety of the methanolic extract of this plant by focusing on its chronic toxicity in mice. For finding chronic toxicity several tests are done such as CBC (Cell Blood count) test and Hepatic enzyme test. All data were analyzed by using SPSS analytical method. Conclusion: After summarize all the results it can say that Mikania cordata may have several pharmacological activities but to prove the hypothesis it need further higher studies