Microbiological analysis of street vended foods from different private universities in Dhaka city

This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.This study was analyzed the microbial quality of street vended food samples. The main objective of this study was to find out the presence of enteric bacteria specially Escherichia coli, Shigella, Vibrios, Klebsiella and Salmonella in different food samples. Total 30 samples were collected from the street vended shops beside the 10 university in Dhaka city, Bangladesh. Five different agar media were used for isolation and identification of our suspected organisms from the samples. The agar media were MacConeyk, TBX (Tryptone Bile X-glucuronide Agar), BGA (Brilliant Green Agar), XLD (Xylose lysine deoxycholate) and TCBS (Thiosulfate citrate-bile salts sucrose). Microbial growth was observed in different media plates. In our present study, 3 suspected organisms E.coli, Klebsiella spp. and Vibio spp. were found from 13 (43%) samples. From the biochemical test results of the colonies of MacConkey, XLD and TCBS agar media, 6 (14%) food samples were suspected to be contaminated with E.coli, 6 (14%) food samples were suspected to be contaminated with Klebsiella spp. and 1 (3%) food sample was suspected to be contaminated with Vibrio spp. No Salmonella spp. was found from any food sample. Seven biochemical tests were performed for characterizing the organisms but PCR test was not done. Therefore it cannot be said confidently that colonies of the media plates are the claimed ones. In this research, due to limitations of facilities other tests were not performed. Therefore, to reduce the frequency of food-borne illness future studies will be needed to determine the presence of various microorganisms responsible for food-borne illnesses