Phytochemical and Biological Investigation of Tridax Procumbens Leaves

This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) of East West University, Dhaka, Bangladesh.The crude methanolic extracts derived from the Tridax procumbens leaves was screened in vitro for possible phytochemical and biological. Crude plant powders were extracted sequentially with methanol and the dried extracts obtained demonstrated the presence of significant pharmacological activity on diabetes, microorganisms responsible for disease. The objective of this study is to characterize the functional compounds that were extracted and separated from leaves of Tridax procumbens and were carried out using different methods using Thin Layer Chromatography (TLC), Vacuum Liquid Chromatography (VLC), Column Chromatography. Under phytochemical analysis, antioxidant test & Chemical screening was done. The antioxidant property found in crude methanolic extracts derived from the Tridax procumbens leaves was very good. A new flavonoid (procumbenetin), isolated from the aerial parts of Tridax procumbens, has been characterised as 3,6-dimethoxy-5,7,2',3',4'-pentahydroxyflavone 7-O-β-D-gluco- pyranoside (1) on the basis of spectroscopic techniques and by chemical means. Tridax procumbens; Flavonoids Plant. Uses in traditional medicine. Commonly used in Indian traditional medicine as anticoagulant, hair tonic, antifungal and insect repellent, in bronchial catarrh, diarrhoea, dysentery, and wound healing. Previously isolated constituents. Alkyl esters, sterols, pentacyclic triterpenes, fatty acids and polysaccharides. New isolated constituent. 3,6-Dimethoxy-5,7,2',3',4'-pentahydroxyflavone 7-O-β- D-glucopyranoside (1), named procumbetin Žyield: 0.016% on dried basis.[citation needed] its is also the best anti coagulant and used to treat the inflamatio