Chemical and Biological Investigations on Chloroform Extract of Spondias Pinnata Bark

This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) of East West University, Dhaka, Bangladesh.The purpose of this study was to understand and investigate the phytochemical contents, cytotoxic effect of the traditional medicinal plant Spondias pinnata. It is classified under the family Anacardiaceae. In this paper the study was conveyed on the chloroform extract of the bark of Spondias pinnata. Many researches were done on this plant species by many scholars at different time period but the intention of the study was to emphasize on the possibility of presence of its antioxidant, cytotoxic property. To prove the cytotoxic property and detect its intensity, the Brine Shrimp Lethality Bio-assay test was done and the result is very significant. Sample LC50 value 4.102 μg/ ml where the standards Tamoxifen show LC50 value 1.142 μg/ ml. To detect the presence of antioxidant property, total phenolic contents, DPPH free radical scavenging activity, was done. DPPH test show IC50 value of Sample is 319.08, where standard Ascorbic acid has IC50 value of 371.18. Total phenolic content test for 250, 200, 150, 100, 50 μg/ml concentration of sample it has X value of 255, 190, 129, 95, 59, where standard Gallic acid contains value 260, 190, 140, 80, 70 mg of GAE (Gallic acid equivalent) per gm of dried extract, respectively. So chloroform extract of Spondias pinnata (bark) have significant amount of phenol. After collection, identification and drying the bark sample, extraction was performed by using chloroform solvent system