Prenatal pesticide exposure andPON1genotype associated with adolescent body fat distribution evaluated by dual X-ray absorptiometry (DXA)

Background Many modern pesticides have endocrine disrupting abilities and early life exposure may affect growth and disease risk later in life. Previously, we reported associations between prenatal pesticide exposure and higher childhood body fat content measured by anthropometry. The associations were affected by child PON1 Q192R genotype. Objective We aimed to study whether prenatal pesticide exposure was still associated with body fat content and distribution in the children at puberty and the potential impact of both maternal and child PON1 Q192R genotype. Methods A prospective cohort study of 247 children born by occupationally exposed or unexposed women (greenhouse workers and controls). Two follow-up examinations (age 10-15 and 11-16 years) included simple anthropometry, skinfold measurements, pubertal staging and blood sampling. Total and regional fat% was determined by dual X-ray absorptiometry (DXA) at age 10- Results Prenatal pesticide exposure was associated with increased total, android and gynoid fat% (DXA) at age 10-15 years after adjustment for sex, socio-economic status and puberty (all0.5 standard deviation score (SDS) p<0.05). Stratified by sex, the associations were significant in girls (total fat: =0.7 SDS, android-gynoid ratio: =0.1, both p<0.05), but not in boys. Carrying the R-allele (child or mother, separately, or both) augmented the differences between exposed and unexposed children (total fat: =1.0 SDS, =0.8 SDS, p<0.05, respectively and =1.2 SDS, p