LOCAL-DISTRIBUTION OF BIOMOLECULES ON BIOSENSING SURFACES INVESTIGATED BY SCANNING FORCE MICROSCOPY

HUBER W, RICHMOND T, Anselmetti D, et al. LOCAL-DISTRIBUTION OF BIOMOLECULES ON BIOSENSING SURFACES INVESTIGATED BY SCANNING FORCE MICROSCOPY. NANOBIOLOGY. 1994;3(3-4):189-200.Systematic imaging of biological macromolecules such as protein A or h-IgG on optical sensor surfaces has been performed using scanning force microscopy (SFM). The SFM measurements provided information on the spatial distribution of biologically active species bound or adsorbed to the surface. They were also used to determine quantitatively the equilibrium surface coverage which is reached in contact with a solution containing a given concentration of biologically active species. These values are compared with the respective values determined by optical surface sensitive measurements