AFFINITY EXTRACTION OF PROTEINS BY MEANS OF REVERSE MICELLAR PHASES CONTAINING A METAL-CHELATING SURFACTANT

POPPENBORG L, Flaschel E. AFFINITY EXTRACTION OF PROTEINS BY MEANS OF REVERSE MICELLAR PHASES CONTAINING A METAL-CHELATING SURFACTANT. BIOTECHNOLOGY TECHNIQUES. 1994;8(5):307-312.The principle of metal chelate-protein interaction was applied for protein extraction by means of reverse micellar phases. For this purpose, an affinity surfactant was synthesized exposing iminodiacetic acid as the hydrophilic moiety. The application of this substance as a cosurfactant leads to enhanced extraction of proteins exhibiting histidine groups on their surface