Analysis of psychotropic medicines bromazepam, nitrazepam and triazolam mixture using thin-layer chromatography and high performance liquid chromatography methods

psychotropic medicines: bromazepam, nitrazepam, and triazolam. analysis using thin layer chromatography and high performance liquid chromatography methods aim: To optimize thin-layer chromatography and high-performance liquid chromatography methods for bromazepam, nitrazepam, triazolam and their mixture qualitative analysis. results: - This mobile phase is suitable for examined substances separation, the qualitative analysis in mixture and determination in pharmaceutical products. -HPLC methodic is suitable for examined substances separation, the qualitative analysis in mixture and determination in pharmaceutical products. methodics: - High performance liquid chromatography(HPLC) : HPLC method bromazepam, nitrazepam, triazolam and their mixture solutions of 0, 1 mg/ml in methanol were analyzed. Chromatograph with a photodiode array detector at wavelength 240 nm range and chromatographic column ACE C18 (5.0 cm x 2.1 mm) with sorbent particle size 5 μm were used for qualitative determination. The analysis was performed by using 0, 1 mol/L sulfuric acid-methanol – acetonitrile (7:8:9) mixture as eluent. Optimized methodic was applied in the analysis of pharmaceutical products “Bromazepam Lannacher” (G.L. Pharma GmbH), “Eunoctin” (Gedeon Richter Plc,), “Halcion” (Pfizer H.C.P) (authorized in LT medicines) test solutions in 0.1% sulphuric acid: ACN solvents. HPLC method was performed using ACE C18 (5.0 cm x 2.1 mm) chromatographic column. Gradient elution mode was used (a mixture of 0, 1% sulfuric acid buffer solvent and ACN solvent – as eluent). Flow rate 1 ml/min. Injection volume 10 μl. Photodiode array detector (200- 600 nm wavelength). Bromazepam, nitrazepam, triazolam absorption of UV light was similar as in scientific literature - Thin layer chromatography (TLC) : For optimization of TLC method bromazepam, nitrazepam, triazolam and their mixture solutions in methanol were analyzed. For mobile phase were used: cyclohexane, methanol, diethylamine, sulfuric acid, acetonitrile (solvents). For spots visualization was used: UV light lamp (254 nm; 365nm). Optimized methodic was applied for pharmaceutical products “Bromazepam Lannacher” (G.L. Pharma GmbH), “Eunoctin” (Gedeon Richter Plc,), “Halcion” (Pfizer H.C.P) (authorized in LT medicines) test solutions in cyclohexane-methanol-diethylamine (75:15:10) solvent system. The best mobile phases for bromazepam, nitrazepam and triazolam mixture qualitative analysis using TLC was: “Cyclohexane-methanol-diethylamine (75:15:10) solvents system with an appropriate rati